Have you ever seen a detective on a crime TV show use DNA analysis to link a culprit to a crime? This technology is based on gel electrophoresis, which uses electricity to separate macromolecules based on time. - Today, we'll show you how to make and use your own gel electrophoresis apparatus. Here's all you need: - A small rectangular plastic container - Gross powder combined with water, measured in a graduated cylinder (instead of DNA) - Food coloring samples to be put in the gel. Make sure to have at least two primary colors and a secondary color. - To separate the colors with electricity, we will need 5 9-volt batteries as the power source for our electric currents. The power will be added to the rectangular container in the form of 2 electrodes placed at either end, made out of stainless steel wire. - We will also need alligator clips with leads to connect our batteries to the electrodes, and finally, some baking soda mixed with water and a buffer solution to run our experiment. - To prepare the buffer solution, add 200 milliliters of bottled water measured using a graduated cylinder, then add 2 grams (or about 1/2 teaspoon) of sodium bicarbonate (baking soda). Mix everything together. - Combine 100 milliliters of your buffer solution with about 1/4 TSP of agarose powder, and microwave the mixture. Carefully pour the hot agarose solution into a container with your three well comb placed about one centimeter from the end. - After about an hour and a half, remove the comb using a butter knife. Cut a space at each end to make room for the electrodes. Insert the electrodes with the leads coming out on the same sides so that you can easily attach the power source. - Pour in the buffer solution (sodium...
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Fingerprint consent for science fair project Form: What You Should Know
Human Informed Consent Form — Blank This form must be signed by a parent (or guardian) of all subjects under 18 Human Informed Consent Form — Word This form must be signed by a parent (or guardian) of all subjects under 18 Human Informed Consent Form — Word & PDF This form must be signed by a parent (or guardian) of all subjects under 18.
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